Tropicalis Team


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Mission

Harland Specific Aims
Progress on Mutagenesis
Mutagenesis Strategies
Sharing Policy
Trop Team

Husbandry

Housing frogs
Raising Tads/Frogs to Adults
Sexing Trops
Lab care

Diseases of X. tropicalis

Database for Frog tracking
Frog Identifcation Tags

Shipping Frogs

Obtain Embryos

hCG injection/Ovulation
Natural Matings
Isolating testis for IVF
Squeezing Females for Eggs
In Vitro Fertilization
De-Jelly Eggs

Sperm Freezing/Storage

Developmental Timetables

Manipulate Embryos

Developmental Timetables

De-Jelly Eggs
Fixation of Embryos

Haploids Protocol
Gynogenesis Protocol

Gamma Mutagenesis
ENU Mutagenesis

Microinjection + timetable
Morpholino Oligos
Transgenics techniques

Visualize Tissues/Genes

In situ hybridization
Ab staining
TUNEL Staining

Cartilage Staining

Sectioning Embryos
H&E Staining of Sections

Tropicalis In situ Library
Tropicalis Plasmid Library

Genetic Techniques

Karyotypes
Cytology

Isolating Genomic DNA
Linkage map@UH

Database for Frog tracking
Frog Identifcation Tags

Sperm Freezing/Storage
Shipping Frogs

Genetic Resources

Inbred lines
Mutants

Tropicalis plasmid library

Genomic Resources

Genome@JGI
Genome Steering Committee Reports (Most Recent)
Metazome

Unique Full Length cDNA Set
Xenopus Ests
EST/cDNA Vector Information
EST Clusters/Full length clones

BAC library@CHORI
WashU Mapping
Linkage map@UH

NIBB XDB (gene expression)
Axeldb (gene expression)

Comparative Genomics LLNL
Genomic Web Sites

Suppliers

Nasco
Pondside Herp Supply
HBH

Aquatic Ecosystems
Marine Biotech

Harland Lab Frogs
Tropicalis Plasmid Library

Tropicalis Web Sites

Trop Labs

Grainger
Amaya
Bronchain
Conlon
Mead
Papalopulu
Stemple
Zimmerman

Harland Lab Tropicalis Team

The Boss:

Richard M. Harland (harland@socrates.berkeley.edu)

The Team (2005):