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Request Frogs

We have a number of frogs availble for distribution. As additional frogs become available we will list them here. To obtain frogs, please send your requests to Richard Harland (harland@socrates.berkeley.edu).

Inbred Strains:

Name Available as: Description
Nigerian mating pair These are inbred Nigerian X. tropicalis kindly provided to us by Rob Grainger and then bred in the lab. We have identified background mutations in these animals - grinch and curly. However, we have identified a set of animals without these background mutations which are available for distribution.
Ivory Coast froglet These are inbred Ivory Coast animals also provided to us by Rob Grainger. They appear to be polymorphic relative to the Nigerian strain. The Ivory Coast and Nigerians are being analyzed to generate a linkage map.
Population A froglets These animals were originally obtained from Rob Grainger and then brought to the Harland lab via David Wright.
Golden froglets The Golden line is being developed in the Harland Lab by Maura Lane. During each generation, she has been aggressively selecting for animals that grow, develop, and reach sexual maturity the fastest. In the latest generation, males have become sexually mature in under 4 months. She is continuing to breed these animals for these characteristics.

Transgenic Strains:

Name Available as: Description
Gamma-crystallin GFP homozygous mating pair These are transgenic frogs derived from the Nigerian line kindly provided to us by Rob Grainger. We have inbred these frogs and now have homozygous gamma-crystallin GFP frogs. These frogs express GFP in the lens of the eye very robustly and is easily scored. We have tested these frogs for curly and grinch and are raising animals that do not appear to be carriers of these background mutations.

Mutants:

Name Available as: Description
grinch heterozygous mating pair grinch is a mutation which appears to affect the circulatory system. Tadpoles develop significant ventral edema but the remainder of the body axis appears quite normal based on gross morphology. grinch matings produce Mendelian ratios expected of a single, recessive, embryonic-lethal allele.
curly heterozygous mating pair curly is a mutation which appears to affect ventral tissues (endoderm) and results in curling of the tail. curly matings produce Mendelian ratios expected of a single, recessive, embryonic-lethal allele.

Tim Grammer, Mustafa Khokha, Maura Lane