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Mission
Harland Specific Aims
Progress on Mutagenesis
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Husbandry
Housing frogs
Raising Tads/Frogs to Adults
Sexing Trops
Lab care
Diseases of X. tropicalis
Database for Frog tracking
Frog Identifcation Tags
S
hipping Frogs
Obtain Embryos
hCG injection/Ovulation
Natural Matings
Isolating testis for IVF
Squeezing Females for Eggs
In Vitro Fertilization
De-Jelly Eggs
Sperm Freezing/Storage
Developmental Timetables
Manipulate
Embryos
Developmental Timetables
De-Jelly Eggs
Fixation of Embryos
Haploids Protocol
Gynogenesis Protocol
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ENU Mutagenesis
Microinjection + timetable
Morpholino Oligos
Transgenics techniques
Visualize
Tissues/Genes
In situ hybridization
Ab staining
TUNEL Staining
Cartilage Staining
Sectioning Embryos
H&E Staining of Sections
Tropicalis In situ Library
Tropicalis Plasmid Library
Genetic
Techniques
Karyotypes
Cytology
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Isolating Genomic DNA
Linkage map@UH
Database for Frog tracking
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Sperm Freezing/Storage
S
hipping Frogs
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Tropicalis plasmid library
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(Most Recent)
Metazome
Unique Full Length cDNA Set
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EST/cDNA Vector Information
EST Clusters/Full length clones
BAC library@CHORI
WashU Mapping
Linkage map@UH
NIBB XDB (gene expression)
Axeldb (gene expression)
Comparative Genomics LLNL
Genomic Web Sites
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Nasco
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Harland Lab Frogs
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Xenopus@NIH
Xenbase
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Grainger
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Conlon
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Stemple
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X. tropicalis Housing Systems
Introduction
Facility
Environment
Tank Systems
Small/Medium Self-Made Tank Systems
Overview
Advantages
Disadvantages
Holding Water System
How to Build the Holding Water System
Drip System
Overview
How to Build the Small and Medium Drip Tank System
The Small and Medium Frog Tanks for Drip System
The Multi-Tank Water Reservoir for the Drip System
How to Build the Drip Line
Large Tank Frog Housing System
Overview
Advantages
Disadvantages
Drip System
Drain System
How to Build the Large Tank System
Aquatic Habitats System
Overview
Advantages
Disadvantages
Marine Biotech System
Overview
Advantages
Disadvantages
Cleaning and Maintenance
Self-Made Systems
Aquatic Habitats System
Useful Supplies
Water Quality Testing
Recordkeeping
Supplier List
These pages contributed by Maura Lane